|
| Status |
Public on Aug 07, 2017 |
| Title |
Myoepithelial_Donor2_day38_rep1 |
| Sample type |
genomic |
| |
|
| Source name |
Myoepithelial
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
transfection: dCas9 3a3l
donor: 2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from myoepithelial cells using Thermo Fisher Scientific PureLink Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
raw data column header: Donor2_p7
|
| Data processing |
ChAMP and minfi packages in R (v.3.3.2 or v.3.4 respectively)
|
| |
|
| Submission date |
Jun 19, 2017 |
| Last update date |
Aug 07, 2017 |
| Contact name |
Emily Saunderson |
| E-mail(s) |
e.saunderson@qmul.ac.uk
|
| Organization name |
Barts Cancer Institute
|
| Street address |
Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE100184 |
Hit-and-run epigenetic editing prevents senescence entry in primary breast cells from healthy donors [EPIC methylation] |
| GSE100209 |
Hit-and-run epigenetic editing prevents senescence entry in primary breast cells from healthy donors |
|
