|
| Status |
Public on Jun 21, 2017 |
| Title |
Il4_1 |
| Sample type |
RNA |
| |
|
| Source name |
Ppard-overexpressing RAW 264.7 cells treated with Il-4
|
| Organism |
Mus musculus |
| Characteristics |
genotype: cell line: Ppard-overexpressing RAW 264.7 cells
phenotype: treated with Il-4
|
| Growth protocol |
Cells were grown in DMEM mudium with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the PrepEase RNA Spin Kit (USB)
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
This sample is of Ppard-overexpressing RAW 264.7 cells treated with Il-4. It is the first of three replicates used in this experiment, each from separate culture.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jun 20, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Lingling Dai |
| Organization name |
Harvard T.H. Chan School of Public Health
|
| Street address |
665 Huntington Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02120 |
| Country |
USA |
| |
|
| Platform ID |
GPL10192 |
| Series (1) |
| GSE100237 |
Gene expression profile of peroxisome proliferator-activated receptor delta (Ppard)-overexpressing RAW 264.7 macrophages treated vehicle, GW501516 or interleukin-4 (Il-4) |
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