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Status |
Public on Jun 21, 2017 |
Title |
Il4_3 |
Sample type |
RNA |
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Source name |
Ppard-overexpressing RAW 264.7 cells treated with Il-4
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Organism |
Mus musculus |
Characteristics |
genotype: cell line: Ppard-overexpressing RAW 264.7 cells phenotype: treated with Il-4
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Growth protocol |
Cells were grown in DMEM mudium with 10% FBS
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the PrepEase RNA Spin Kit (USB)
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Label |
Cy3
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Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
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Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
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Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
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Description |
This sample is of Ppard-overexpressing RAW 264.7 cells treated with Il-4. It is the third of three replicates used in this experiment, each from separate culture.
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Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4 (Roche NimbleGen, Inc.).
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Submission date |
Jun 20, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Lingling Dai |
Organization name |
Harvard T.H. Chan School of Public Health
|
Street address |
665 Huntington Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02120 |
Country |
USA |
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Platform ID |
GPL10192 |
Series (1) |
GSE100237 |
Gene expression profile of peroxisome proliferator-activated receptor delta (Ppard)-overexpressing RAW 264.7 macrophages treated vehicle, GW501516 or interleukin-4 (Il-4) |
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