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Sample GSM2676099 Query DataSets for GSM2676099
Status Public on Jun 21, 2017
Title Il4_3
Sample type RNA
 
Source name Ppard-overexpressing RAW 264.7 cells treated with Il-4
Organism Mus musculus
Characteristics genotype: cell line: Ppard-overexpressing RAW 264.7 cells
phenotype: treated with Il-4
Growth protocol Cells were grown in DMEM mudium with 10% FBS
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the PrepEase RNA Spin Kit (USB)
Label Cy3
Label protocol Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
 
Hybridization protocol Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Description This sample is of Ppard-overexpressing RAW 264.7 cells treated with Il-4. It is the third of three replicates used in this experiment, each from separate culture.
Data processing The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4 (Roche NimbleGen, Inc.).
 
Submission date Jun 20, 2017
Last update date Jan 23, 2018
Contact name Lingling Dai
Organization name Harvard T.H. Chan School of Public Health
Street address 665 Huntington Avenue
City Boston
State/province MA
ZIP/Postal code 02120
Country USA
 
Platform ID GPL10192
Series (1)
GSE100237 Gene expression profile of peroxisome proliferator-activated receptor delta (Ppard)-overexpressing RAW 264.7 macrophages treated vehicle, GW501516 or interleukin-4 (Il-4)

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
AB000096 1616.4172
AB000490 347.2508
AB001425 23512.6149
AB001435 1985.8858
AB001539 35817.1006
AB001750 43435.6343
AB001926 42190.4392
AB003502 52998.5126
AB004048 13456.6526
AB005662 25193.6235
AB005665 411.7827
AB005909 1969.0323
AB006034 696.0774
AB006103 415.4301
AB007407 1723.9589
AB008928 1569.166
AB009369 1290.549
AB010088 348.7543
AB010122 55943.0189
AB011499 46175.171

Total number of rows: 44170

Table truncated, full table size 832 Kbytes.




Supplementary file Size Download File type/resource
GSM2676099_KSTAA09.pair.gz 2.8 Mb (ftp)(http) PAIR
Processed data included within Sample table

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