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Sample GSM267838 Query DataSets for GSM267838
Status Public on May 15, 2008
Title MuRF1 transgene_1
Sample type RNA
 
Source name M. quadriceps
Organism Mus musculus
Characteristics strain: FVBN
genotype/variation: MuRF1 overexpression
gender: male
age: 3 month
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from skeletal muscles using RNeasy mini kit (Qiagen) according to manufacturer’s protocol.
Label biotin
Label protocol Between 15 to 40 ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
 
Hybridization protocol Hybridization was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Scan protocol Scanning was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Description TG/WT at 12 weeks of age were compared.
Data processing quantile normalization (SAS GEnomics 3.0)
 
Submission date Feb 25, 2008
Last update date Nov 30, 2010
Contact name Stephanie Hirner
E-mail(s) stephanie.hirner@anaes.ma.uni-heidelberg.de
Phone 0049-(0)621-3831626
Organization name institute of anaesthesiology and intensive care
Department Universitätsklinikum Mannheim
Street address Theodor-Kutzer-Ufer1-3
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platform ID GPL6526
Series (1)
GSE10626 MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies

Data table header descriptions
ID_REF
VALUE normalized intensities (quantile normalization)

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 7.42297191
AFFX-18SRNAMur/X00686_5_at 7.27101994
AFFX-18SRNAMur/X00686_M_at 7.00079423
AFFX-BioB-3_at 12.1997834
AFFX-BioB-5_at 11.9431351
AFFX-BioB-M_at 10.6585289
AFFX-BioC-3_at 7.1430752
AFFX-BioC-5_at 7.14439407
AFFX-BioDn-3_at 7.30508761
AFFX-BioDn-5_at 8.25221517
AFFX-CreX-3_at 8.03128142
AFFX-CreX-5_at 10.5478149
AFFX-DapX-3_at 9.41988221
AFFX-DapX-5_at 11.7990894
AFFX-DapX-M_at 11.8913522
AFFX-GapdhMur/M32599_3_at 11.8730137
AFFX-GapdhMur/M32599_5_at 10.3375128
AFFX-GapdhMur/M32599_M_at 11.4719961
AFFX-LysX-3_at 13.2175375
AFFX-LysX-5_at 13.1713056

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM267838.CEL.gz 5.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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