|
| Status |
Public on May 15, 2008 |
| Title |
MuRF1 transgene_2 |
| Sample type |
RNA |
| |
|
| Source name |
TG2 M. quadriceps
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVBN
genotype/variation: MuRF1 overexpression
gender: male
age: 3 month
tissue: skeletal muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from skeletal muscles using RNeasy mini kit (Qiagen) according to manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Between 15 to 40 ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
|
| Scan protocol |
Scanning was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
|
| Description |
TG/WT at 12 weeks of age were compared.
|
| Data processing |
quantile normalization (SAS GEnomics 3.0)
|
| |
|
| Submission date |
Feb 25, 2008 |
| Last update date |
Nov 30, 2010 |
| Contact name |
Stephanie Hirner |
| E-mail(s) |
stephanie.hirner@anaes.ma.uni-heidelberg.de
|
| Phone |
0049-(0)621-3831626
|
| Organization name |
institute of anaesthesiology and intensive care
|
| Department |
Universitätsklinikum Mannheim
|
| Street address |
Theodor-Kutzer-Ufer1-3
|
| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6526 |
| Series (1) |
| GSE10626 |
MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies |
|
