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Sample GSM267839 Query DataSets for GSM267839
Status Public on May 15, 2008
Title MuRF1 transgene_2
Sample type RNA
 
Source name TG2 M. quadriceps
Organism Mus musculus
Characteristics strain: FVBN
genotype/variation: MuRF1 overexpression
gender: male
age: 3 month
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from skeletal muscles using RNeasy mini kit (Qiagen) according to manufacturer’s protocol.
Label biotin
Label protocol Between 15 to 40 ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
 
Hybridization protocol Hybridization was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Scan protocol Scanning was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Description TG/WT at 12 weeks of age were compared.
Data processing quantile normalization (SAS GEnomics 3.0)
 
Submission date Feb 25, 2008
Last update date Nov 30, 2010
Contact name Stephanie Hirner
E-mail(s) stephanie.hirner@anaes.ma.uni-heidelberg.de
Phone 0049-(0)621-3831626
Organization name institute of anaesthesiology and intensive care
Department Universitätsklinikum Mannheim
Street address Theodor-Kutzer-Ufer1-3
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platform ID GPL6526
Series (1)
GSE10626 MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies

Data table header descriptions
ID_REF
VALUE normalized intensities (quantile normalization)

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 7.53185789
AFFX-18SRNAMur/X00686_5_at 6.8818088
AFFX-18SRNAMur/X00686_M_at 6.74677978
AFFX-BioB-3_at 12.2241244
AFFX-BioB-5_at 11.8752457
AFFX-BioB-M_at 10.7831807
AFFX-BioC-3_at 7.4173895
AFFX-BioC-5_at 7.37162195
AFFX-BioDn-3_at 7.64723462
AFFX-BioDn-5_at 8.50273803
AFFX-CreX-3_at 8.33075747
AFFX-CreX-5_at 10.7035317
AFFX-DapX-3_at 9.60150347
AFFX-DapX-5_at 11.8689601
AFFX-DapX-M_at 11.9147068
AFFX-GapdhMur/M32599_3_at 12.5035045
AFFX-GapdhMur/M32599_5_at 11.5959953
AFFX-GapdhMur/M32599_M_at 12.4463109
AFFX-LysX-3_at 13.1879323
AFFX-LysX-5_at 13.1327772

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM267839.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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