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Sample GSM267840 Query DataSets for GSM267840
Status Public on May 15, 2008
Title WT1
Sample type RNA
 
Source name WT1 M. quadriceps
Organism Mus musculus
Characteristics strain: FVBN
genotype/variation: wildtype
gender: male
age: 3 month
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from skeletal muscles using RNeasy mini kit (Qiagen) according to manufacturer’s protocol.
Label biotin
Label protocol Between 15 to 40 ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
 
Hybridization protocol Hybridization was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Scan protocol Scanning was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Description TG/WT at 12 weeks of age were compared.
Data processing quantile normalization (SAS GEnomics 3.0)
 
Submission date Feb 25, 2008
Last update date Nov 30, 2010
Contact name Stephanie Hirner
E-mail(s) stephanie.hirner@anaes.ma.uni-heidelberg.de
Phone 0049-(0)621-3831626
Organization name institute of anaesthesiology and intensive care
Department Universitätsklinikum Mannheim
Street address Theodor-Kutzer-Ufer1-3
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platform ID GPL6526
Series (1)
GSE10626 MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies

Data table header descriptions
ID_REF
VALUE normalized intensities (quantile normalization)

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 6.93036251
AFFX-18SRNAMur/X00686_5_at 6.3000561
AFFX-18SRNAMur/X00686_M_at 6.41352563
AFFX-BioB-3_at 12.1634543
AFFX-BioB-5_at 11.8390628
AFFX-BioB-M_at 10.5733719
AFFX-BioC-3_at 6.90835315
AFFX-BioC-5_at 7.00406642
AFFX-BioDn-3_at 7.09202658
AFFX-BioDn-5_at 8.02855364
AFFX-CreX-3_at 7.84998743
AFFX-CreX-5_at 10.221002
AFFX-DapX-3_at 9.0621305
AFFX-DapX-5_at 11.4924816
AFFX-DapX-M_at 11.5057838
AFFX-GapdhMur/M32599_3_at 11.8469705
AFFX-GapdhMur/M32599_5_at 10.6076695
AFFX-GapdhMur/M32599_M_at 11.7161729
AFFX-LysX-3_at 13.1368648
AFFX-LysX-5_at 13.0785544

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM267840.CEL.gz 5.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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