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Sample GSM267841 Query DataSets for GSM267841
Status Public on May 15, 2008
Title WT2
Sample type RNA
 
Source name M. quadriceps
Organism Mus musculus
Characteristics strain: FVBN
genotype/variation: wildtype
gender: male
age: 3 month
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from skeletal muscles using RNeasy mini kit (Qiagen) according to manufacturer’s protocol.
Label biotin
Label protocol Between 15 to 40 ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
 
Hybridization protocol Hybridization was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Scan protocol Scanning was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
Description TG/WT at 12 weeks of age were compared.
Data processing quantile normalization (SAS GEnomics 3.0)
 
Submission date Feb 25, 2008
Last update date Nov 30, 2010
Contact name Stephanie Hirner
E-mail(s) stephanie.hirner@anaes.ma.uni-heidelberg.de
Phone 0049-(0)621-3831626
Organization name institute of anaesthesiology and intensive care
Department Universitätsklinikum Mannheim
Street address Theodor-Kutzer-Ufer1-3
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platform ID GPL6526
Series (1)
GSE10626 MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies

Data table header descriptions
ID_REF
VALUE normalized intensities (quantile normalization)

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 9.26997596
AFFX-18SRNAMur/X00686_5_at 8.52003367
AFFX-18SRNAMur/X00686_M_at 7.94317463
AFFX-BioB-3_at 11.8712125
AFFX-BioB-5_at 11.6482928
AFFX-BioB-M_at 9.86793163
AFFX-BioC-3_at 7.41815513
AFFX-BioC-5_at 7.41304112
AFFX-BioDn-3_at 7.53084535
AFFX-BioDn-5_at 8.48123483
AFFX-CreX-3_at 8.28209782
AFFX-CreX-5_at 10.6308867
AFFX-DapX-3_at 9.59243452
AFFX-DapX-5_at 11.9080937
AFFX-DapX-M_at 11.9138437
AFFX-GapdhMur/M32599_3_at 12.258292
AFFX-GapdhMur/M32599_5_at 11.1243417
AFFX-GapdhMur/M32599_M_at 12.1457079
AFFX-LysX-3_at 13.1081205
AFFX-LysX-5_at 13.1250311

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM267841.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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