|
| Status |
Public on Feb 28, 2008 |
| Title |
Human ileum crypt epithelium P2 |
| Sample type |
RNA |
| |
|
| Source name |
Ileal tissue biopsies
|
| Organism |
Homo sapiens |
| Characteristics |
Sample was obtained from individual undergoing surgery for colon cancer, bowel obstruction, or other non-inflammatory conditions. Fixed overnight in 4% (w/v) paraformaldehyde
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA extraction protocol with proteinase K digestion of cellular extract
|
| Label |
biotin
|
| Label protocol |
Affymetrix 2-cycle amplification and labeling protocol
|
| |
|
| Hybridization protocol |
16 hr at 55rpm in an Affymetrix hybridization oven at 45C
|
| Scan protocol |
100 format, Affymetrix scanner 3000
|
| Description |
The specimens were free of macroscopic signs of disease or inflammation
|
| Data processing |
GCOS
|
| |
|
| Submission date |
Feb 25, 2008 |
| Last update date |
Feb 27, 2008 |
| Contact name |
Michael George |
| E-mail(s) |
mdgeorge@ucdavis.edu
|
| Organization name |
University of California, Davis
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
451 Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE10629 |
Human ileum epithelium LCMD Project |
|
