|
Status |
Public on Dec 01, 2017 |
Title |
Alveolar macrophages from control mice_AM_Control_1 |
Sample type |
RNA |
|
|
Source name |
Alveolar macrophages from control mice
|
Organism |
Mus musculus |
Characteristics |
strain background: C57Bl/6 animal identification number: Control C57Bl/6 mouse 1 treated with: none (control) tissue: Murine alveolar macrophage
|
Treatment protocol |
Control mice underwent oropharyngeal instillation of 35% surfactant/saline and experimental animals were exposed to 35% surfactant/saline solution containing 100 μg of MWCNT.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from macrophages was isolated and purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
|
Label |
Biotin
|
Label protocol |
High quality RNA samples were converved to cDNA and biotin-labaled
|
|
|
Hybridization protocol |
Hybridization to microarrays were performed per Illumina protocols.
|
Scan protocol |
Illumina iScan system
|
Data processing |
Microarray data was assessed for quality, followed by variance stabilization and quantile normalization using the Bioconductor package lumi.
|
|
|
Submission date |
Jun 26, 2017 |
Last update date |
Oct 19, 2022 |
Contact name |
Sina Gharib |
E-mail(s) |
sagharib@uw.edu
|
Organization name |
University of Washington
|
Department |
Medicine
|
Street address |
850 Republican St.
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109 |
Country |
USA |
|
|
Platform ID |
GPL6887 |
Series (1) |
GSE100500 |
Transcriptional Survey of Alveolar Macrophages in a Murine Model of Chronic Granulomatous Inflammation Reveals Common Themes with Human Sarcoidosis |
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