|
| Status |
Public on Dec 01, 2017 |
| Title |
Alveolar macrophages from MWCNT-exposed mice_AM_MWCNT_3 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar macrophages from MWCNT-exposed mice
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
animal identification number: MWCNT C57Bl/6 mouse 3
treated with: multiwall carbon nanotube (MWCNT)
tissue: Murine alveolar macrophage
|
| Treatment protocol |
Control mice underwent oropharyngeal instillation of 35% surfactant/saline and experimental animals were exposed to 35% surfactant/saline solution containing 100 μg of MWCNT.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from macrophages was isolated and purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
|
| Label |
Biotin
|
| Label protocol |
High quality RNA samples were converved to cDNA and biotin-labaled
|
| |
|
| Hybridization protocol |
Hybridization to microarrays were performed per Illumina protocols.
|
| Scan protocol |
Illumina iScan system
|
| Data processing |
Microarray data was assessed for quality, followed by variance stabilization and quantile normalization using the Bioconductor package lumi.
|
| |
|
| Submission date |
Jun 26, 2017 |
| Last update date |
Oct 19, 2022 |
| Contact name |
Sina Gharib |
| E-mail(s) |
sagharib@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Medicine
|
| Street address |
850 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE100500 |
Transcriptional Survey of Alveolar Macrophages in a Murine Model of Chronic Granulomatous Inflammation Reveals Common Themes with Human Sarcoidosis |
|
