|
| Status |
Public on Jan 01, 2018 |
| Title |
untreated total RNA |
| Sample type |
RNA |
| |
|
| Source name |
LN229 untreated total RNA fraction
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LN229
|
| Treatment protocol |
untreated or ATO (5 micromolar) for 6 hours in DMEM
|
| Growth protocol |
LN229 cells were grown in DMEM with 10% FBS and gentamycin in the presence or absence of ATO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed using hypotonic lysis buffer containing cyclohexamide. Polysomal RNA was isolated by ultracentrifugation and fractionation using a sucrose gradient. Total and polysomal RNA fractions were processed using the AllPrep kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
The manufacturer's instructions were followed to synthesize and label ss-cDNA using the Affymetric GeneChip WT Reagent kit.
|
| |
|
| Hybridization protocol |
GeneChip Hybridization, Wash & Stain Kit according to manufacturer’s instructions. GeneChips were washed and stained by the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data files were produced using the Affymetrix Expression Console. The values represent the average of three replicates per group.
|
| |
|
| Submission date |
Jun 27, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Matthew J. Schipma |
| E-mail(s) |
m-schipma@northwestern.edu
|
| Phone |
1-312-503-5324
|
| Organization name |
Northwestern University
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
750 N. Lake Shore Drive
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE100523 |
Gene expression from total and polysomal RNA fractions after ATO treatment in LN229 cells. |
|
