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Sample GSM2702271 Query DataSets for GSM2702271
Status Public on Apr 17, 2019
Title ExVivoColon_DNBS+L. paracasei_rep2
Sample type RNA
 
Source name ExVivoColon_DNBS+L. paracasei
Organism Mus musculus
Characteristics tissue: Colon
age: 10w
gender: Male
strain: C57BL/5
treatment: DNBS+L. paracasei
replicate: 2
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from total tissue of colon by using the column-based RNeasy® Micro Kit (Qiagen). RNA was quantified using a NanoDrop-1000 spectrophotometer and RNA quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Samples with RNA integrity number (RIN) ranging from 8.5-10 were selected for further analysis.
Label Cy3
Label protocol Labelling of total RNA (100ng) was performed with the One-Color Low Input Quick Amp Labelling kit (Agilent Technologies).
 
Hybridization protocol Samples were hybridized to SurePrint G3 mouse 8x60K v2 (G4858A-074809) arrays and scanned by G2565CA Scanner System (Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 1x60k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
Description Gene expression after 23 days of treatment by DNBS+L. paracasei
Sample name: 17.4
Data processing Image analyses and raw signal intensities were quantified by Feature Extraction Software v10.7.3.1 (Agilent Technologies) according to the GE1_107_Sep09 protocol.
 
Submission date Jul 13, 2017
Last update date Apr 17, 2019
Contact name Rebeca Martin Rosique
E-mail(s) rbkmai@hotmail.com
Organization name INRA
Department MICALIS
Lab Probiotic commensal host interactions laboratory
Street address Domaine de Vilvert bat 440 R-2
City Jouy en Josas
State/province Yvelines
ZIP/Postal code 78350
Country France
 
Platform ID GPL21163
Series (1)
GSE101411 Transcriptomic analysis of mice treated with Lactobacillus rhamnosus CNCM i-3690 and different mutants and variants generated from this strain

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
(+)E1A_r60_1 19.07273254
(+)E1A_r60_3 5.755761294
(+)E1A_r60_a104 6.444373372
(+)E1A_r60_a107 8.632183382
(+)E1A_r60_a135 11.33659018
(+)E1A_r60_a20 13.05577469
(+)E1A_r60_a22 14.1500619
(+)E1A_r60_a97 16.19394219
(+)E1A_r60_n11 18.46742261
(+)E1A_r60_n9 18.62037833
3xSLv1 5.349376573
A_30_P01017428 7.372272534
A_30_P01017437 7.925970182
A_30_P01017440 7.256495285
A_30_P01017441 5.708963943
A_30_P01017444 5.736072972
A_30_P01017445 6.325909216
A_30_P01017447 8.066838918
A_30_P01017448 5.596315319
A_30_P01017453 5.375922971

Total number of rows: 56745

Table truncated, full table size 1411 Kbytes.




Supplementary file Size Download File type/resource
GSM2702271_257480910766_201602261008_S01_GE1_107_Sep09_1_2.txt.gz 12.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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