|
| Status |
Public on Jun 14, 2018 |
| Title |
DNAM-1- NK subsets from 17Day AML Spleen |
| Sample type |
SRA |
| |
|
| Source name |
DNAM-1- NK cells, AML spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
age: 6-8 weeks
time point post-transplantation: day 17
tissue: AML spleen
cell type: DNAM-1- natural killer cells
|
| Treatment protocol |
GFP+ leukemia cells were isolated from leukemia mice and transplanted into 6-8 weeks old C57BL/6J mice (1×10e6 cells/mouse) without irradiation. All mice suffered AML with a survival time of 35 to 40 days. DNAM-1+ and DNAM-1- NK cells were sorted from spleen of leukemia mice at 0d and 17d.
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted DNAM-1+ and DNAM-1- NK cells were harvested and resuspended in 350ul RLT and 3.5ul β-Me. Total RNA was exacted by QIAGEN RNeasy Mini Kit (CAT 74103).
1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard BGISEQ-500 protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
S4
|
| Data processing |
Base calling was done by BGISEQ-500.
Read pairs with low quality in either read were filtered out (10% bases with N or 50% bases with Q5).
Alignment was performed against RefSeq by bowtie2 (version 2.2.5).
FPKM values of each gene for each sample were measured via RSEM (version v1.2.12).
Genome_build: RefSeq
Supplementary_files_format_and_content: *txt: Tab-delimited text files include FPKMs.
|
| |
|
| Submission date |
Jul 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Guo Guang Zheng |
| E-mail(s) |
zhenggg@ihcams.ac.cn
|
| Phone |
+86-02223909402
|
| Organization name |
Chinese Academy of Medical Science& Peking Union Medical College
|
| Department |
Institute of Hematology & Blood Hospital
|
| Lab |
State Key Laboratory of Hematology
|
| Street address |
Blood Hospital,288 Nanjing Road,Heping District
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE101421 |
Next-generation sequencing facilitates quantitative analysis of DNAM-1+ and DNAM-1- natural killer (NK) cells transcriptomes in mouse MLL-AF9 AML leukemia |
|
| Relations |
| BioSample |
SAMN07350503 |
| SRA |
SRX3003640 |
