|
| Status |
Public on Mar 08, 2008 |
| Title |
Exp A, Animal #249, 500mg/kg D-Serine |
| Sample type |
RNA |
| |
|
| Source name |
Brain tissue from Fisher 344 male rat, Animal #249, 500mg/kg D-Serine
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sex: male
Age: adult (11-12 weeks)
|
| Treatment protocol |
Male Fisher 344 rats aged 11-12 weeks were treated with various doses (0, 5, 20, 50, 200 & 500 mg/kg) of d-serine and terminally sacrificed 96 hours post-exposure. An approximate 30mg-section of the forebrain was processed for total RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from brain tissue using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA). Frozen tissues were homogenized in lysis buffer containing guanidinium thiocyanate and -mercaptoethanol. Extraction and purification of total RNA from tissue
|
| Label |
biotin
|
| Label protocol |
Microarrays were stained in the Affymetrix GeneChip Fluidics Station 450 using the EukGE_Ws2v5 protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed at 45°C for 16 h in an Affymetrix hybridization oven with constant end over end rotation at 60 RPM.
|
| Scan protocol |
GeneChips were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GeneChip Operating Software (GCOS, version 1.2).
|
| Description |
Gene expression data from male F344 rats treated with a single dose of 500mg/kg D-Serine for 96 hours
|
| Data processing |
Data obtained from the Affymetrix GeneChip Scanner 3000 was processed using the statistical algorithm of the Affymetrix GCOS. The gene expression signal was scaled using "All Probe Sets Scaling" with a target intensity of 500.
|
| |
|
| Submission date |
Mar 06, 2008 |
| Last update date |
Mar 07, 2008 |
| Contact name |
Molly Davidson |
| E-mail(s) |
molly.davidson@wpafb.af.mil
|
| Organization name |
AFRL/RHPB
|
| Street address |
2729 R Street, Bldg 837
|
| City |
WPAFB |
| State/province |
OH |
| ZIP/Postal code |
45433 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE10748 |
Expression data from brain tissue of Rattus norvegicus treated with D-Serine |
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