Strain: C57BL6 Heart:ventricles and septum, Female total RNA (MoHe.FeVS) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoHe.FeVS.sig21 Cell type Heart:ventricles and septum, Female Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoHe.FeVS_sig21.5044T.d-20 7/12/2004 447604 7493 QC Passed MoHe.FeVS_sig21.5044W.c-20 6/21/2004 525751 8421 QC Passed MoHe.FeVS_sig21.5044W.b-20 6/18/2004 529209 8021 QC Passed MoHe.FeVS_sig21.5044T.b-20 6/14/2004 376452 7247 QC Passed MoHe.FeVS_sig21.5044T.a-20 6/11/2004 392258 7934 QC Passed MoHe.FeVS_sig21.5044W.a-20 6/9/2004 530837 9781 QC Passed Run group: Total Beads successfully sequenced - 2802111 Processed Signatures - 14461