NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM27141 Query DataSets for GSM27141
Status Public on Oct 26, 2004
Title Kidney:medulla, Female (MoKi.FeMd)
Sample type MPSS
 
Source name Kidney:medulla, Female total RNA
Organism Mus musculus
Extracted molecule total RNA
 
Description Strain: C57BL6
Kidney:medulla, Female total RNA (MoKi.FeMd) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Cells/tissue:
Library MoKi.FeMd.sig21
Cell type Kidney:medulla, Female
Source NHGRI
RNA isolation LYNX mRNA QC passed
cDNA library:
Library DpnII restriction - (signature cloning using MmeI)
Sequence length 20 bp
MPSS:
runs
MoKi.FeMd_sig21.5088T.b-20 7/8/2004 334137 7742 QC Passed
MoKi.FeMd_sig21.5088T.a-20 7/1/2004 360439 8237 QC Passed
MoKi.FeMd_sig21.5088W.a-20 7/1/2004 523286 11119 QC Passed
MoKi.FeMd_sig21.5088W.b-20 7/1/2004 468659 11631 QC Passed
MoKi.FeMd_sig21.5032T.c-20 6/4/2004 377661 6834 QC Passed
MoKi.FeMd_sig21.5032W.a-20 5/28/2004 578888 11639 QC Passed
MoKi.FeMd_sig21.5032T.a-20 5/27/2004 371621 6469 QC Passed
Run group:
Total Beads successfully sequenced - 3014691
Processed Signatures - 17060
 
Submission date Jul 20, 2004
Last update date Dec 14, 2005
Contact name Daixing Zhou
E-mail(s) dzhou@solexa.com
Phone 510-670-9441
Fax 510-670-9302
URL http://www.solexa.com
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
 
Platform ID GPL1010
Series (1)
GSE1581 MPSS mouse transcriptome analysis project

Data table header descriptions
ID_REF Signature - 20 nt sequence including DpnII site (GATC)
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global


Total number of rows: 9217

Table truncated, full table size 273 Kbytes.




Supplementary file Size Download File type/resource
GSM27141.raw.txt.gz 250.3 Kb (ftp)(http) TXT

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap