Strain: C57BL6 Kidney:contex, Male total RNA (MoKi.MaCr) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoKi.MaCr.sig21 Cell type Kidney:contex, Male Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoKi.MaCr_sig21.5001T.d-20 6/3/2004 447120 11030 QC Passed MoKi.MaCr_sig21.5001W.e-20 5/27/2004 533136 13173 QC Passed MoKi.MaCr_sig21.5001W.d-20 5/19/2004 521427 15116 QC Passed MoKi.MaCr_sig21.5001T.c-20 5/17/2004 397844 9288 QC Passed MoKi.MaCr_sig21.5001T.a-20 5/17/2004 425707 8591 QC Passed MoKi.MaCr_sig21.5001W.b-20 5/14/2004 652448 14229 QC Passed Run group: Total Beads successfully sequenced - 2977682 Processed Signatures - 21011
