|
| Status |
Public on Jul 27, 2017 |
| Title |
10 uM Enzalutamide+0.1 nM R1881 |
| Sample type |
RNA |
| |
|
| Source name |
10 uM Enzalutamide+0.1 nM R1881
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
agent: 10 uM Enzalutamide+0.1 nM R1881
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from LNCaP cells using the qiagen RNA isolation kit and its quality was verified using the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Clariom®D Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
|
| Description |
gene expression data human tissue
|
| Data processing |
The data were analyzed with affmetrix expression console default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jul 26, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Daniel Lee Johnson |
| E-mail(s) |
djohn166@uthsc.edu
|
| Phone |
9014483743
|
| Organization name |
UTHSC
|
| Department |
Molecular Informatics Core
|
| Street address |
71 S Manassas
|
| City |
Memphis |
| State/province |
Tennessee |
| ZIP/Postal code |
38163 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE101897 |
Effect of Selective Androgen Receptor Degraders (SARDs) on Androgen Receptor (AR) Function in LNCaP Cells |
|
