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Status |
Public on Jul 27, 2017 |
Title |
10 uM UT-69+0.1 nM R1881 |
Sample type |
RNA |
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Source name |
10 uM UT-69+0.1 nM R1881
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Organism |
Homo sapiens |
Characteristics |
cell line: LNCaP agent: 10 uM UT-69+0.1 nM R1881
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from LNCaP cells using the qiagen RNA isolation kit and its quality was verified using the Agilent 2100 Bioanalyzer (Agilent Technologies).
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Clariom®D Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
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Description |
gene expression data human tissue
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Data processing |
The data were analyzed with affmetrix expression console default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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Submission date |
Jul 26, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Daniel Lee Johnson |
E-mail(s) |
djohn166@uthsc.edu
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Phone |
9014483743
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Organization name |
UTHSC
|
Department |
Molecular Informatics Core
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Street address |
71 S Manassas
|
City |
Memphis |
State/province |
Tennessee |
ZIP/Postal code |
38163 |
Country |
USA |
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Platform ID |
GPL23159 |
Series (1) |
GSE101897 |
Effect of Selective Androgen Receptor Degraders (SARDs) on Androgen Receptor (AR) Function in LNCaP Cells |
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