|
| Status |
Public on Mar 08, 2008 |
| Title |
IRAK1/IRAK2 KO_macrophages_2h |
| Sample type |
RNA |
| |
|
| Source name |
IRAK1/IRAK2 KO macrophages stimulated with MALP-2 for 2 h
|
| Organism |
Mus musculus |
| Characteristics |
IRAK1/IRAK2 KO macrophages stimulated with MALP-2 for 2 h
|
| Treatment protocol |
stimulated with 100 ng/ml MALP-2 for indicated periods
|
| Growth protocol |
peritoneal macrophages were preprared from thioglycollate-injected mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared by NuGEN Ovation system according to the manufactuerer's inscrution.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
slides were scanned using the Affymetrix GeneChip scanner 3000.
|
| Description |
Gene expression data from IRAK1/IRAK2 KO macrophages stimulated with MALP-2 for 2h
|
| Data processing |
The data were analyzed with RMA using the arrayassist program (Stratagene).
|
| |
|
| Submission date |
Mar 07, 2008 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Osamu Takeuchi |
| E-mail(s) |
otake@mfour.med.kyoto-u.ac.jp
|
| Phone |
81-75-753-9500
|
| Organization name |
Kyoto University
|
| Department |
Grad School of Medicine
|
| Lab |
Medical Chemistry
|
| Street address |
Yoshida-konoe-cho, Sakyo-ku,
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10765 |
Expression data from MALP-2-stimulated macrophages from wild-type, IRAK-2-/- and IRAK-1-/IRAK-2-/- mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
