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Status
Public on Nov 05, 2004
Title
INS-1 cell line (FLP-In T-REx clone #1-1.16)
Sample type
RNA
Source name
INS-1 cell line, clone #1-1.16
Organism
Rattus norvegicus
Extracted molecule
total RNA
Description
Starting with the rat insulinoma cell line INS-1, clones were generated that contain a FRT site for site specific integration of specific genes (INS-1 Flp-In T-REx). In addition, the clones contain the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factor can be identified. This entry corresponds to the RNA isolated from the INS-1 cell line #1-1.16 Keywords = INS-1 Keywords = b-cell Keywords = Flp-In T-Rex Keywords = tetracycline Keywords = FRT
Submission date
Jul 22, 2004
Last update date
May 27, 2005
Contact name
Ludger Klein-Hitpass
E-mail(s)
ludger.klein-hitpass@uni-essen.de
Phone
+49 201 723 85552
Organization name
Institut fuer Zellbiologie
Department
Universitaetsklinikum
Lab
BioChip Lab
Street address
Virchowstr. 173
City
Essen
ZIP/Postal code
D-45122
Country
Germany
Platform ID
GPL341
Series (2)
GSE1591
INS-1 cell lines (FLP-In T-REx)
GSE6428
HNF1b, HNF4a2 and HNF6 targets
Data table header descriptions
ID_REF
VALUE
‘signal’, a measure of the abundance of the transcript
ABS_CALL
the call in an absolute analysis that indicates whether a transcript is reliably detected (Present, P) or not detected (Absent, A)
DETECTION P-VALUE
p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
359.9
P
0.003212
AFFX-BioB-M_at
489.2
P
0.000509
AFFX-BioB-3_at
189.7
P
0.012547
AFFX-BioC-5_at
635.7
P
0.000127
AFFX-BioC-3_at
478
P
0.00007
AFFX-BioDn-5_at
1142.4
P
0.000446
AFFX-BioDn-3_at
4886.2
P
0.000127
AFFX-CreX-5_at
8363.6
P
0.000044
AFFX-CreX-3_at
12908.9
P
0.000044
AFFX-DapX-5_at
131
P
0.015183
AFFX-DapX-M_at
393
P
0.012547
AFFX-DapX-3_at
356.6
P
0.00141
AFFX-LysX-5_at
876.4
P
0.000044
AFFX-LysX-M_at
1190.4
P
0.000044
AFFX-LysX-3_at
2404.9
P
0.000044
AFFX-PheX-5_at
4899.9
P
0.000044
AFFX-PheX-M_at
5788
P
0.000044
AFFX-PheX-3_at
6656.3
P
0.000044
AFFX-ThrX-5_at
715.7
P
0.000081
AFFX-ThrX-M_at
978.3
P
0.000044
Total number of rows: 15923 Table truncated, full table size 432 Kbytes .Supplementary data files not provided