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Sample GSM2722381 Query DataSets for GSM2722381
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-Mac donor 1
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 1
treatment: IL-34 + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.80680951172443
7892502 4.69605801752964
7892503 2.81162564064159
7892504 7.09397263793666
7892505 2.99263690498694
7892506 4.93519722606166
7892507 4.22853847972397
7892508 7.22734175302709
7892509 11.053289216936
7892510 6.52738549959972
7892511 5.24421278769728
7892512 4.32082284834963
7892513 2.98419098747086
7892514 10.5484055484534
7892515 8.82803504169443
7892516 4.14474626154195
7892517 5.55094134063399
7892518 3.45269646284089
7892519 3.81716792983857
7892520 8.72539453330485

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722381_IS09_ARN0038_s1h2_B05_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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