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Sample GSM2722383 Query DataSets for GSM2722383
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-Mac donor 1
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 1
treatment: MSCF + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.21320910988335
7892502 3.95301301123917
7892503 2.76140185754366
7892504 7.09046332237354
7892505 3.13757072537859
7892506 3.73474733021127
7892507 3.88903683543206
7892508 6.85983179520209
7892509 10.9494567782852
7892510 6.45530064671791
7892511 5.67140542395996
7892512 4.51351206976059
7892513 3.097129259104
7892514 10.182011169739
7892515 8.98019001119691
7892516 4.32984563944505
7892517 5.77434347265307
7892518 3.05512849499143
7892519 4.30732587626149
7892520 8.71631807326542

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722383_IS09_ARN0040_s1h2_D05_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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