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Sample GSM2722384 Query DataSets for GSM2722384
Status Public on Sep 15, 2017
Title GMCSF + IL-4 mo-DC donor 1
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 1
treatment: GMCSF + IL-4
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.14786718464858
7892502 4.85430370489553
7892503 3.09238963840878
7892504 7.02755545397432
7892505 3.05346548223208
7892506 4.72928344249162
7892507 3.52293159736198
7892508 6.83244238352441
7892509 10.8440611585361
7892510 6.74797669429973
7892511 4.84738099467536
7892512 4.78746809212167
7892513 2.97145128014475
7892514 10.0080425253092
7892515 8.87616532778739
7892516 3.627789649778
7892517 5.51953886736794
7892518 3.15155699122673
7892519 3.9333669284256
7892520 8.68101134903709

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722384_IS09_ARN0041_s1h2_E05_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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