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Sample GSM2722385 Query DataSets for GSM2722385
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-DC donor 2
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 2
treatment: IL-34 + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.83529452110717
7892502 4.85550012206073
7892503 3.04785738902355
7892504 7.29765087899328
7892505 3.01898736168976
7892506 5.30552702635314
7892507 3.76014658962877
7892508 7.00821088858956
7892509 10.8019574170746
7892510 7.17242218133109
7892511 5.13984399174669
7892512 4.78329290835792
7892513 3.2405481076694
7892514 9.99889302987281
7892515 8.96840828243057
7892516 4.20169138183352
7892517 6.12292720337656
7892518 3.40116953224406
7892519 3.69987731297944
7892520 8.60660983780544

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722385_IS09_ARN0042_s1h2_F05_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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