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Sample GSM2722386 Query DataSets for GSM2722386
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-Mac donor 2
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 2
treatment: IL-34 + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.11613532558153
7892502 4.67378707623428
7892503 2.94698704684987
7892504 7.13601469119942
7892505 3.21342038441853
7892506 5.13180391583401
7892507 4.39858478634929
7892508 7.1361058320169
7892509 10.8929055941409
7892510 6.83390449027312
7892511 5.71330390395488
7892512 4.6361353358325
7892513 3.26016354765698
7892514 10.3903386650951
7892515 9.474722915562
7892516 3.92248104277407
7892517 6.3037317163891
7892518 3.30372803925099
7892519 4.37347396889561
7892520 8.80967745399261

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722386_IS09_ARN0043_s1h2_G05_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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