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Sample GSM2722388 Query DataSets for GSM2722388
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-Mac donor 2
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 2
treatment: MSCF + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.55905764753862
7892502 4.35334291310342
7892503 2.85873971772752
7892504 7.16838033407152
7892505 3.13503338185722
7892506 5.1552438247547
7892507 3.93315811535845
7892508 6.55239671596698
7892509 10.840837295645
7892510 6.19221272413379
7892511 5.26526408063084
7892512 4.13991406969664
7892513 3.0749143020072
7892514 10.5531032822957
7892515 9.25682338312043
7892516 3.64603894698974
7892517 6.02065989732974
7892518 3.68197079027849
7892519 3.89357153802611
7892520 8.81514912589873

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722388_IS09_ARN0045_s1h2_A07_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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