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Sample GSM2722389 Query DataSets for GSM2722389
Status Public on Sep 15, 2017
Title GMCSF + IL-4 mo-DC donor 2
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 2
treatment: GMCSF + IL-4
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.81945873989865
7892502 5.08290668634191
7892503 3.26635713465397
7892504 6.8343222909592
7892505 3.31326427989324
7892506 4.92228597289648
7892507 3.7705612047582
7892508 6.85547570857585
7892509 10.9057809213333
7892510 6.77171846598991
7892511 5.38702376045685
7892512 4.78979728063982
7892513 3.16539715280189
7892514 10.005280171893
7892515 8.95264675892397
7892516 3.61917945436234
7892517 6.20886638177966
7892518 3.42630841882123
7892519 4.12924569506152
7892520 8.53439683066402

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722389_IS09_ARN0046_s1h2_B07_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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