|
| Status |
Public on Sep 15, 2017 |
| Title |
GMCSF + IL-4 mo-DC donor 2 |
| Sample type |
RNA |
| |
|
| Source name |
healthy donor monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
individual: donor 2
treatment: GMCSF + IL-4
cell type: mo-DC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAEasy micro-kit
|
| Label |
biotin
|
| Label protocol |
100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
|
| |
|
| Hybridization protocol |
Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
|
| |
|
| Submission date |
Jul 31, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Elodie Segura |
| E-mail(s) |
elodie.segura@curie.fr
|
| Phone |
+336156246720
|
| Organization name |
Institut Curie
|
| Department |
INSERM U932
|
| Street address |
26 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE102046 |
Transcriptomic analysis of in vitro-generated human monocyte-derived cells |
|
