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Sample GSM2722390 Query DataSets for GSM2722390
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-DC donor 3
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 3
treatment: IL-34 + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.51813375526534
7892502 4.27218135142208
7892503 2.64604331499759
7892504 6.75141986457856
7892505 3.26945378107728
7892506 4.68829588925447
7892507 4.11739266793152
7892508 7.28237117569145
7892509 11.0860861283471
7892510 6.55047683447857
7892511 5.8478970002392
7892512 5.00390659103859
7892513 3.1012432288658
7892514 10.389880810911
7892515 8.68164633312026
7892516 3.91838396705127
7892517 5.62192301441816
7892518 3.40246236037995
7892519 3.75268024196198
7892520 8.46590354211971

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722390_IS09_ARN0047_s1h2_C07_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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