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Sample GSM2722392 Query DataSets for GSM2722392
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-DC donor 3
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 3
treatment: MSCF + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.45575020724579
7892502 4.4134911572739
7892503 3.10923509394409
7892504 6.72176639608066
7892505 3.12358155366621
7892506 4.92606893470865
7892507 4.34022140312855
7892508 7.12693426419808
7892509 11.0996900196836
7892510 6.54675637738384
7892511 5.18841171756367
7892512 4.88703679266411
7892513 3.01598245892652
7892514 10.2181775403342
7892515 8.8957109123715
7892516 4.87023925370696
7892517 6.39165229896052
7892518 3.29863783618251
7892519 4.19439313243323
7892520 8.73576440933799

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722392_IS09_ARN0049_s1h2_E07_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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