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Sample GSM2722393 Query DataSets for GSM2722393
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-Mac donor 3
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 3
treatment: MSCF + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.622260776403
7892502 4.72930996082228
7892503 2.9222293889941
7892504 6.97509627591969
7892505 3.33782482850293
7892506 4.92462426807429
7892507 4.67606767723522
7892508 6.84862611675657
7892509 10.6673334875893
7892510 6.57209912511567
7892511 5.4742776793211
7892512 5.01552421014888
7892513 3.48120694473796
7892514 10.2708295830282
7892515 9.02244729368175
7892516 3.90347747749295
7892517 5.54238826454639
7892518 3.78436730713842
7892519 4.40071513123159
7892520 8.66082911255805

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722393_IS09_ARN0050_s1h2_F07_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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