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Sample GSM2722394 Query DataSets for GSM2722394
Status Public on Sep 15, 2017
Title GMCSF + IL-4 mo-DC donor 3
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 3
treatment: GMCSF + IL-4
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.9563726284852
7892502 4.88584752812671
7892503 2.87914276718353
7892504 6.76800744123137
7892505 3.10847396082281
7892506 5.49629774832538
7892507 3.96572337685104
7892508 7.05498027140038
7892509 10.9060462785237
7892510 6.67151381863001
7892511 5.15302170919249
7892512 4.3379195315182
7892513 3.47145229018758
7892514 10.096578524905
7892515 8.91922087507396
7892516 3.9199536707993
7892517 5.90011817806771
7892518 3.27985390959254
7892519 4.08195484242265
7892520 8.49001110889447

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722394_IS09_ARN0051_s1h2_G07_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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