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Sample GSM2722395 Query DataSets for GSM2722395
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-DC donor 4
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 4
treatment: IL-34 + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.54369721737809
7892502 5.12498413702195
7892503 2.89486211913643
7892504 7.28964716287199
7892505 3.05305776254552
7892506 5.12017033313199
7892507 3.85791676146906
7892508 7.0542290329676
7892509 10.6243412877686
7892510 6.60264143093194
7892511 5.96595042781215
7892512 4.77046462094887
7892513 3.56174307682791
7892514 10.234732969112
7892515 9.08540235871711
7892516 4.28030985015647
7892517 5.83388744849406
7892518 3.1328475959945
7892519 4.08527267750357
7892520 8.66610512521568

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722395_IS09_ARN0052_s1h2_H07_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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