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Sample GSM2722396 Query DataSets for GSM2722396
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-Mac donor 4
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 4
treatment: IL-34 + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.32912729368809
7892502 4.62061870429795
7892503 3.27310298055262
7892504 7.22534724313024
7892505 3.00418191609752
7892506 5.70237136700823
7892507 4.64939935196537
7892508 6.55467351280382
7892509 10.3602873104619
7892510 6.4495947218154
7892511 5.75284277498039
7892512 4.95461695217126
7892513 3.20184989547
7892514 10.7169409122396
7892515 9.13790178267679
7892516 4.1192774237878
7892517 5.63751621151113
7892518 2.77935044535227
7892519 4.46974753220407
7892520 8.25095180068189

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722396_IS09_ARN0053_s1h1_A05_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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