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Sample GSM2722397 Query DataSets for GSM2722397
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-DC donor 4
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 4
treatment: MSCF + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.44801051085074
7892502 4.54763516294257
7892503 2.72179338038619
7892504 7.3976844166556
7892505 3.00908529782757
7892506 4.93487510830337
7892507 4.83385882917413
7892508 7.14172761943777
7892509 10.6069958230892
7892510 6.5929785335934
7892511 6.04812849979951
7892512 5.03026609331026
7892513 3.18884439743597
7892514 10.3090294382621
7892515 9.1728851690531
7892516 3.99777481244387
7892517 5.891540399965
7892518 2.97000476120743
7892519 4.13374047774575
7892520 8.46906040372312

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722397_IS09_ARN0054_s1h1_B05_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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