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Sample GSM2722399 Query DataSets for GSM2722399
Status Public on Sep 15, 2017
Title GMCSF + IL-4 mo-DC donor 4
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 4
treatment: GMCSF + IL-4
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.15573744073387
7892502 4.68810719895317
7892503 3.22659532150218
7892504 6.57532729815898
7892505 3.38870212475428
7892506 5.96602783627036
7892507 4.44483760645261
7892508 6.89397765591542
7892509 10.6276742942667
7892510 5.81609250235752
7892511 4.85110349801998
7892512 4.03274673368881
7892513 2.96039551633863
7892514 10.3684859247784
7892515 8.91941718398307
7892516 4.32246227081057
7892517 6.04461796692094
7892518 2.74379792739455
7892519 4.59848652172806
7892520 8.81371728556699

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722399_IS09_ARN0056_s1h1_D05_hGene.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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