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Sample GSM2722401 Query DataSets for GSM2722401
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-Mac donor 5
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 5
treatment: IL-34 + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.17539597638204
7892502 4.4813383650464
7892503 2.56408545031006
7892504 6.98220837888924
7892505 2.97942453500421
7892506 4.76082535268456
7892507 4.32140790228142
7892508 6.92974858013182
7892509 10.5475291124742
7892510 5.94509622221667
7892511 5.71670540267527
7892512 5.02524285772803
7892513 2.91210844411316
7892514 10.6297742933633
7892515 8.91739812626916
7892516 3.33485792143523
7892517 6.12986470875023
7892518 3.0461331589811
7892519 5.01984724035977
7892520 8.37723181792993

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722401_IS09_ARN0058_s1h1_F05_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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