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Sample GSM2722402 Query DataSets for GSM2722402
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-DC donor 5
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 5
treatment: MSCF + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.65563477155922
7892502 4.12302903328468
7892503 3.25917052395946
7892504 6.7888311645243
7892505 2.77737410248161
7892506 5.60507626749837
7892507 3.64547697661389
7892508 6.50937812770358
7892509 10.7614551223446
7892510 5.75574645449383
7892511 5.06769463216066
7892512 4.71799587314063
7892513 3.12769282872088
7892514 10.2402700762731
7892515 8.84353869473157
7892516 3.62544922226395
7892517 4.80566288321221
7892518 2.97054383527623
7892519 4.56188569834859
7892520 8.68352775353627

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722402_IS09_ARN0059_s1h1_G05_hGene.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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