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Sample GSM2722403 Query DataSets for GSM2722403
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-Mac donor 5
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 5
treatment: MSCF + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.0599437821165
7892502 4.33820181448033
7892503 3.01545318291056
7892504 6.71794426926247
7892505 2.66595559617694
7892506 4.5470254344732
7892507 4.188737369427
7892508 6.77632367524996
7892509 10.8132126630402
7892510 6.21080341627103
7892511 5.56319491606659
7892512 4.38933950653303
7892513 3.11322528820173
7892514 10.5806511564789
7892515 8.56656002333798
7892516 4.26537398382632
7892517 5.28486219584125
7892518 2.9728435542011
7892519 3.9977572990516
7892520 8.30731354851749

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722403_IS09_ARN0060_s1h1_H05_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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