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Sample GSM2722404 Query DataSets for GSM2722404
Status Public on Sep 15, 2017
Title GMCSF + IL-4 mo-DC donor 5
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 5
treatment: GMCSF + IL-4
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.10898824382183
7892502 4.0006266695899
7892503 2.83764798541722
7892504 6.6338570891291
7892505 2.95761126084477
7892506 4.72880572211888
7892507 3.44599858787124
7892508 6.54896515619545
7892509 10.529952069975
7892510 6.21611166549
7892511 4.92293781289391
7892512 5.39474855925337
7892513 2.88830917996232
7892514 10.0743599463541
7892515 9.03627820490275
7892516 4.28711714934329
7892517 5.88652112759495
7892518 3.1289041593925
7892519 4.64587311836254
7892520 8.69100505004044

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722404_IS09_ARN0061_s1h1_A07_hGene.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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