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Sample GSM2722405 Query DataSets for GSM2722405
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-DC donor 6
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 6
treatment: IL-34 + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.27312407750962
7892502 4.23192599579001
7892503 2.66577114215137
7892504 7.2246848612373
7892505 3.08247881312403
7892506 4.20556758277965
7892507 4.46900067937316
7892508 6.76465783826877
7892509 10.2903465240202
7892510 6.40709170621529
7892511 5.80081958069891
7892512 4.94602742672218
7892513 3.40328539005735
7892514 10.5373073364327
7892515 9.13712028928743
7892516 4.82291843686132
7892517 5.38928296052439
7892518 2.85646755181023
7892519 4.52184798439336
7892520 8.54676335523603

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722405_IS09_ARN0062_s1h1_B07_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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