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Sample GSM2722406 Query DataSets for GSM2722406
Status Public on Sep 15, 2017
Title IL-34 + IL-4 + TNFa mo-Mac donor 6
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 6
treatment: IL-34 + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 4.34033330135583
7892502 3.969491146117
7892503 2.66069209357709
7892504 7.49428012588108
7892505 2.98186341953116
7892506 5.70001523415525
7892507 4.08704753241413
7892508 6.60177843283536
7892509 10.1863483621754
7892510 6.22238584042872
7892511 5.85137497695611
7892512 5.91706911417653
7892513 2.82119355549238
7892514 10.3641875044601
7892515 9.17679014010952
7892516 3.87264753044116
7892517 5.73814070944592
7892518 3.28151532885997
7892519 5.0010848608527
7892520 8.62309864954665

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722406_IS09_ARN0063_s1h1_C07_hGene.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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