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Sample GSM2722407 Query DataSets for GSM2722407
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-DC donor 6
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 6
treatment: MSCF + IL-4 + TNFa
cell type: mo-DC
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.91389421422766
7892502 4.65745384030612
7892503 2.67739777426999
7892504 7.37434596078511
7892505 3.03188464897351
7892506 4.78734307015482
7892507 4.50234783573967
7892508 7.19359529848577
7892509 10.4461450379763
7892510 6.52537738436132
7892511 5.88282017651036
7892512 5.06792709248511
7892513 2.85980073445506
7892514 10.3568729981539
7892515 9.04887963011812
7892516 4.10658002455044
7892517 5.46168106808377
7892518 2.82663538316881
7892519 5.10227822737891
7892520 8.58949487675783

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722407_IS09_ARN0064_s1h1_D07_hGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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