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Sample GSM2722408 Query DataSets for GSM2722408
Status Public on Sep 15, 2017
Title MSCF + IL-4 + TNFa mo-Mac donor 6
Sample type RNA
 
Source name healthy donor monocytes
Organism Homo sapiens
Characteristics individual: donor 6
treatment: MSCF + IL-4 + TNFa
cell type: mo-Mac
Extracted molecule total RNA
Extraction protocol Qiagen RNAEasy micro-kit
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
 
Submission date Jul 31, 2017
Last update date Jan 23, 2018
Contact name Elodie Segura
E-mail(s) elodie.segura@curie.fr
Phone +336156246720
Organization name Institut Curie
Department INSERM U932
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE102046 Transcriptomic analysis of in vitro-generated human monocyte-derived cells

Data table header descriptions
ID_REF
VALUE log (2) RMA signal

Data table
ID_REF VALUE
7892501 3.50092043980906
7892502 4.2081636253724
7892503 2.72417832035945
7892504 8.00401618755851
7892505 2.99990213963034
7892506 4.95090613857723
7892507 4.05699247768326
7892508 6.07877625536674
7892509 10.2373704659967
7892510 6.23247460571859
7892511 5.38547918824621
7892512 5.61562096155372
7892513 2.91550821056594
7892514 10.7454884235613
7892515 9.12156469941089
7892516 3.8042761034129
7892517 6.15972169599204
7892518 3.0755219636117
7892519 4.54510747685199
7892520 8.56611171325211

Total number of rows: 33297

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM2722408_IS09_ARN0065_s1h1_E07_hGene.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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