|
| Status |
Public on Sep 18, 2018 |
| Title |
Empty VECTOR #1 |
| Sample type |
RNA |
| |
|
| Source name |
mice 9393P cells, empty VECTOR
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 9393P
stable transfection: VECTOR
|
| Treatment protocol |
Cells were transfected using jetPRIME® Versatile DNA/siRNA transfection reagent (Polyplus). Stable cell transfectants were selected with puromycin (InvivoGene) or G418 (Corning).
|
| Growth protocol |
All cell lines were maintained in RPMI supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell lines by the RNeasy Mini Kit (Qiagen). RNA quality and quantity were evaluated on a NanoDrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
RNA fluorescent labeling reaction and hybridization were performed using the Affymetrix (Clariom S WT Plus) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
According to the manufacturer’s instructions.
|
| Scan protocol |
According to the manufacturer’s instructions.
|
| Description |
393P-VEC-1
Mice 9393P cells with VECTOR, sample 1.
|
| Data processing |
Expression arrays were processed and RMA normalized using BioConductor.
|
| |
|
| Submission date |
Aug 02, 2017 |
| Last update date |
Sep 18, 2018 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE102168 |
Gene expression profiling of AC9 overexpression in 393P mice cells |
|
