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Status |
Public on Sep 18, 2018 |
Title |
Empty VECTOR #2 |
Sample type |
RNA |
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Source name |
mice 9393P cells, empty VECTOR
|
Organism |
Mus musculus |
Characteristics |
cell line: 9393P stable transfection: VECTOR
|
Treatment protocol |
Cells were transfected using jetPRIME® Versatile DNA/siRNA transfection reagent (Polyplus). Stable cell transfectants were selected with puromycin (InvivoGene) or G418 (Corning).
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Growth protocol |
All cell lines were maintained in RPMI supplemented with 10% fetal bovine serum.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from cell lines by the RNeasy Mini Kit (Qiagen). RNA quality and quantity were evaluated on a NanoDrop spectrophotometer.
|
Label |
Cy3
|
Label protocol |
RNA fluorescent labeling reaction and hybridization were performed using the Affymetrix (Clariom S WT Plus) according to the manufacturer’s instructions.
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|
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Hybridization protocol |
According to the manufacturer’s instructions.
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Scan protocol |
According to the manufacturer’s instructions.
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Description |
393P-VEC-2 Mice 9393P cells with VECTOR, sample 2.
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Data processing |
Expression arrays were processed and RMA normalized using BioConductor.
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Submission date |
Aug 02, 2017 |
Last update date |
Sep 18, 2018 |
Contact name |
Chad Creighton |
E-mail(s) |
creighto@bcm.tmc.edu
|
Organization name |
Baylor College of Medicine
|
Department |
Biostatistics, Ducan Cancer Center
|
Street address |
One Baylor Plaza, Mail Stop: BCM305
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE102168 |
Gene expression profiling of AC9 overexpression in 393P mice cells |
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