|
| Status |
Public on Nov 22, 2020 |
| Title |
Subject 20, timepoint WK0 |
| Sample type |
RNA |
| |
|
| Source name |
Plasma microRNA sample for subject 20 at timepoint WK0
|
| Organism |
Homo sapiens |
| Characteristics |
timepoint: WK0
tissue: plasma
subject id: Subject 27
response: Good
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNA was extracted by Assurgen using a proprietary method. cDNA was synthesized with Taqman miRNA Reverse Transcription kit (Thermofisher Scientific) in conjunction with Megaplex RT primers (Pools A & B, Cat #4399966 & # 4444281). cDNA targets were further pre-amplified using Megaplex Preamp Primers (Pool A, Cat # 4399233 & Pool B, Cat # 4444303) & mixed with Taqman OA Real-time PCR Master Mix following the manufacturer's protocol and loaded onto OpenArray Human miRNA plates (Cat # 4461104) using an AccuFill (Applied Biosystems). miRNA panels were run on a QuantStudio 12K Flex instrument.
|
| Label |
not provided
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
5703-00803_WK0
|
| Data processing |
The raw data were processed using a custom R script, and normalized using Delta-CT with geNorm. MicroRNAs absent in all samples were discarded.
|
| |
|
| Submission date |
Aug 04, 2017 |
| Last update date |
Nov 22, 2020 |
| Contact name |
Ilya Korsunsky |
| E-mail(s) |
ikorsuns@broadinstitute.org
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Genetics
|
| Lab |
Raychaudhuri
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL17485 |
| Series (1) |
| GSE102263 |
Change in circulating miRNA at two weeks after anti-TNF therapy predicts response at 14 weeks: a pilot study |
|
