|
| Status |
Public on Oct 01, 2017 |
| Title |
[S100β-v-erbB/p53-/- TSC1 cell]-[Hypoxia+ DMSO] 3 |
| Sample type |
RNA |
| |
|
| Source name |
S100β-v-erbB/p53-/- cells culture
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB-NJ
agent: Hypoxia+ DMSO
|
| Treatment protocol |
Cell cultures were treated with either DMSO control, BFA (1uM), or EHT-1864 (1uM) grown in hypoxia or normoxia (16h).
|
| Growth protocol |
Cell cultures were grown in DMEM F-12 50-50 media supplemented with B27.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol mRNA extraction
|
| Label |
biotin
|
| Label protocol |
WTPLus kit (by Affymetrix)
|
| |
|
| Hybridization protocol |
GeneChipTM Hybridization, Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
GeneChipTM Scanner 3000
|
| Data processing |
Raw data generated from Clariom S Mouse Arrays was processed using Affymetrix Expression Console Software. CEL files containing feature intensity values were converted into summarized expression values using a robust multichip analysis (RMA) which consists of background adjustment, quantile normalization and summarization across all chips.
|
| |
|
| Submission date |
Aug 24, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Damian A Almiron Bonnin |
| E-mail(s) |
dalmironbonnin@gmail.com
|
| Organization name |
Geisel School of Medicine at Dartmouth College
|
| Department |
Molecular and Systems Biology
|
| Lab |
Laboratory of Dr. Mark Israel
|
| Street address |
1 Medical Center Drive
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE103022 |
Microarray of S100β-v-erbB/p53-/- cells cultured in normoxia, hypoxia, hypoxia with Brefeldin A (0.1uM) and hypoxia EHT-1864 (1uM). |
|
