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Status |
Public on Oct 01, 2017 |
Title |
[S100β-v-erbB/p53-/- TSC1 cell]-[Hypoxia+ BFA] 1 |
Sample type |
RNA |
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Source name |
S100β-v-erbB/p53-/- cells culture
|
Organism |
Mus musculus |
Characteristics |
strain: FVB-NJ agent: Hypoxia+ BFA
|
Treatment protocol |
Cell cultures were treated with either DMSO control, BFA (1uM), or EHT-1864 (1uM) grown in hypoxia or normoxia (16h).
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Growth protocol |
Cell cultures were grown in DMEM F-12 50-50 media supplemented with B27.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol mRNA extraction
|
Label |
biotin
|
Label protocol |
WTPLus kit (by Affymetrix)
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Hybridization protocol |
GeneChipTM Hybridization, Wash and Stain Kit (Affymetrix)
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Scan protocol |
GeneChipTM Scanner 3000
|
Data processing |
Raw data generated from Clariom S Mouse Arrays was processed using Affymetrix Expression Console Software. CEL files containing feature intensity values were converted into summarized expression values using a robust multichip analysis (RMA) which consists of background adjustment, quantile normalization and summarization across all chips.
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Submission date |
Aug 24, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Damian A Almiron Bonnin |
E-mail(s) |
dalmironbonnin@gmail.com
|
Organization name |
Geisel School of Medicine at Dartmouth College
|
Department |
Molecular and Systems Biology
|
Lab |
Laboratory of Dr. Mark Israel
|
Street address |
1 Medical Center Drive
|
City |
Lebanon |
State/province |
NH |
ZIP/Postal code |
03756 |
Country |
USA |
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Platform ID |
GPL23038 |
Series (1) |
GSE103022 |
Microarray of S100β-v-erbB/p53-/- cells cultured in normoxia, hypoxia, hypoxia with Brefeldin A (0.1uM) and hypoxia EHT-1864 (1uM). |
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