|
| Status |
Public on Jan 01, 2018 |
| Title |
cord blood 1 whole |
| Sample type |
genomic |
| |
|
| Source name |
cord blood
|
| Organism |
Homo sapiens |
| Characteristics |
individual: 1
sample type: WholeBloodDNA
slide id: 200989060190
Sex: M
race: White
gestational age (weeks): 39.57
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the DNeasy Blood kit (Qiagen) per manufacturer instructions
|
| Label |
biotin and dinitrophenyl
|
| Label protocol |
Illumina HD Methylation protocol
|
| |
|
| Hybridization protocol |
DNA was bisulfite treated and cleaned using the EZ DNA methylation kit (Zymo Research), then hybridized on the Infinium MethylationEPIC BeadChip (Illumina)
|
| Scan protocol |
Methylation NXT scan setting with Illumina iScan
|
| Data processing |
Raw image array files were processed in R statistical software (version 3.3) using the minfi package (version 1.20.2). Data were processed with noob background correction. Probes that failed detection p > 0.01 in 1 or more samples were dropped (n = 2,699). An additional 43,096 probes annotated as cross reactive were also removed.
|
| |
|
| Submission date |
Aug 28, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Kelly Bakulski |
| E-mail(s) |
bakulski@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Department of Epidemiology
|
| Street address |
1415 Washington Heights
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE103189 |
Cord blood buffy coat DNA methylation is comparable to whole cord blood methylation |
|
